Medical science advances at an astonishing pace on all fronts. The area of diagnostics is one particular field where great efforts are made to devise tests and procedures that can help doctors to diagnose diseases quicker and more accurately. This is important because successful treatment often depends upon an early diagnosis. ELISA kits, for example, have empowered doctors to efficiently detect a wide range of diseases.
This revolutionary technique, also known as EIA, or enzyme immunoassay, is conducted in a laboratory. It is used to determine the concentration of antigens or antibodies in a specific sample. The presence and concentration of antibodies is an important indication of the presence of a variety of medical conditions. The test is extremely popular because it is easy to do and many different tests can be processed at the same time.
The presence of antigens or antibodies, or both, in the body is a clear sign of disease. This is because antigens are known as harmful substances. The body is able to detect the presence of antigens and it then immediately start producing antibodies to fight the antigens. It is therefore logical to assume that the presence of either substance indicates the presence of disease.
Patients need not fear this simple test. It only entails the drawing of a small blood sample. This sample is then added to specific antigens in a petri dish. If there is a reaction between the blood sample and the antigen, then the presence of antibodies is confirmed. In fact, the antibodies will combine with the antigens. By adding enzymes it is possible to determine the amount of the antibodies in the sample.
The EIA test provides a wealth of information, even when administered in its simplest form. However, more complex techniques can provide even better results. Different formats, or kits, are used for the different forms of the test. For example, in the direct EIA test the antigen is coated with a multiwall plate. The antibody can only detect it if the latter has been conjugated with an enzyme.
The indirect IEA test is another example of how this protocol can be used in an advanced manner. In this instance the antigen is actually covered by a polystyrene plate. This forces the antibodies to go through two distinct stages before the antigen is detected. This test allows technicians to identify both primary and secondary antibodies. This is valuable because it may eliminate the need for further expensive tests.
In the case of yet another type of EIA test, the sandwich test, pairs of antibodies are tested. The first antibody is covered with a polystyrene plate. The next antibody is then added in order to determine the concentration of the analyte. This is a complicated procedure but it is extremely accurate and flexible.
Nobody will deny the fact that one of the most important components of medical treatment is the ability to diagnose diseases. The earlier it is done the easier it is to treat and the better the prognosis will be. EIA offers hope to patients and it enables physicians to offer better chances.
This revolutionary technique, also known as EIA, or enzyme immunoassay, is conducted in a laboratory. It is used to determine the concentration of antigens or antibodies in a specific sample. The presence and concentration of antibodies is an important indication of the presence of a variety of medical conditions. The test is extremely popular because it is easy to do and many different tests can be processed at the same time.
The presence of antigens or antibodies, or both, in the body is a clear sign of disease. This is because antigens are known as harmful substances. The body is able to detect the presence of antigens and it then immediately start producing antibodies to fight the antigens. It is therefore logical to assume that the presence of either substance indicates the presence of disease.
Patients need not fear this simple test. It only entails the drawing of a small blood sample. This sample is then added to specific antigens in a petri dish. If there is a reaction between the blood sample and the antigen, then the presence of antibodies is confirmed. In fact, the antibodies will combine with the antigens. By adding enzymes it is possible to determine the amount of the antibodies in the sample.
The EIA test provides a wealth of information, even when administered in its simplest form. However, more complex techniques can provide even better results. Different formats, or kits, are used for the different forms of the test. For example, in the direct EIA test the antigen is coated with a multiwall plate. The antibody can only detect it if the latter has been conjugated with an enzyme.
The indirect IEA test is another example of how this protocol can be used in an advanced manner. In this instance the antigen is actually covered by a polystyrene plate. This forces the antibodies to go through two distinct stages before the antigen is detected. This test allows technicians to identify both primary and secondary antibodies. This is valuable because it may eliminate the need for further expensive tests.
In the case of yet another type of EIA test, the sandwich test, pairs of antibodies are tested. The first antibody is covered with a polystyrene plate. The next antibody is then added in order to determine the concentration of the analyte. This is a complicated procedure but it is extremely accurate and flexible.
Nobody will deny the fact that one of the most important components of medical treatment is the ability to diagnose diseases. The earlier it is done the easier it is to treat and the better the prognosis will be. EIA offers hope to patients and it enables physicians to offer better chances.
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